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Quantitative measurement of green fluorescent protein expression |
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| Authors: | C. Renee Albano Lisa Randers-Eichhon Qing Chang William E. Bentley Govind Rao |
| Affiliation: | (1) Molecular and Cellular Biology Program, University of Maryland Baltimore County, 21250 Baltimore, Maryland, USA;(2) Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, 21250 Baltimore, Maryland, USA;(3) Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, USA;(4) Department of Chemical Engineering, University of Maryland College Park, 20742 College Park, Maryland, USA;(5) Medical Biotechnology Center of the Maryland Biotechnology Institute, University of Maryland, 21201 Baltimore, Maryland, USA |
| Abstract: | Summary Data presented here shows a time course analysis of E. coli shake flask cultures expressing the reporter gene green fluorescent protein (GFP) with simultaneous comparison of microbial fluorescence intensity measurements and GFP concentration measured by Western blot. There is an apparent lag between the presence of GFP and its fluorescence due to the time required for formation of the chromophore. We demonstrate that GFP fluorescence can be used as a quantifiable reporter gene, provided the cyclization time for chromophore formation is considered. |
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