Synchrotron X-ray micro-computed tomography as a tool for in situ elucidation of insect bacteriomes |
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| Affiliation: | 1. Agricultural Research Organization, Gilat Research Center, D.N. Negev 85280, Israel;2. Museum für Naturkunde, Leibniz Institute for Research on Evolution and Biodiversity at the Humboldt University Berlin, Invalidenstrasse 43, 10115 Berlin, Germany;3. Agricultural Research Organization, Newe Ya''ar Research Center, Ramat Yishay, Israel;1. Norwegian University of Science and Technology, Department of Psychology, Neuroscience Unit, 7491 Trondheim, Norway;2. Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences, Pr. Torez 44, 194223 Saint-Petersburg, Russia;1. Ernst-Moritz-Arndt-Universität Greifswald, Zoologisches Institut und Museum, Abteilung Cytologie und Evolutionsbiologie, Soldmannstr. 23, D-17487 Greifswald, Germany;2. Universität Rostock, Institut für Biowissenschaften, Lehrstuhl für Allgemeine & Spezielle Zoologie, Universitätsplatz 2, D-18051 Rostock, Germany;3. Universität Duisburg-Essen, Universitätsklinikum Essen, Zentrales Tierlaboratorium, Hufelandstr. 55, D-45122 Essen, Germany;1. Zoologische Staatssammlung München, Münchhausenstraße 21, 81247 München, Germany;2. Department Biologie II, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany;1. Department of Biology, Universitätsstraße 10, University of Konstanz, Konstanz 78457, Germany;2. Life Science Division, Max Schmidheiny-Strasse 201, Leica Microsystems (Schweiz) AG, Heerbrugg, Switzerland;3. Department of Behavioral Physiology and Sociobiology, Biozentrum, Am Hubland, University of Würzburg, Würzburg, Germany;4. Computational and Evolutionary Biology, Faculty of Life Science, University of Manchester, Manchester, United Kingdom;1. Institut für Spezielle Zoologie und Evolutionsbiologie, FSU Jena, 07743 Jena, Germany;2. Department of Entomology, Biological Faculty, Lomonosov Moscow State University, Leninskie gory 1-12, Moscow, Russia;3. New Zealand Arthropod Collection, Private Bag 92170, Auckland, New Zealand;1. Humboldt-Universität zu Berlin, Institut für Biologie/Vergleichende Zoologie, Philippstrasse 13, D-10115 Berlin, Germany;2. Raffles Museum of Biodiversity Research, Department of Biological Sciences, Faculty of Science, National University of Singapore, 6 Science Drive 2, Singapore 117546, Singapore;3. Landcare Research, 231 Morrin Rd, Tamaki Campus, University of Auckland, Private Bag 92170, Auckland, New Zealand |
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| Abstract: | Obligate bacterial endosymbionts are common, influential associates of arthropods, and are often found in specific organs termed bacteriomes. Three dimensional images of bacteriomes of the leafhopper Orosius albicinctus (Hemiptera: Cicadellidae) were reconstructed from synchrotron-based X-ray micro-computed tomography (CT). Results show that bilateral bacteriomes are located between the first and second abdominal tergites, are mushroom-shaped and consist two different types of tissue. Fluorescence in situ hybridization reveals that the primary bacterial symbiont Sulcia muelleri is in the ‘cap’ part of the of organ. The technique allows a noninvasive, in situ, means of visualizing bacteriomes and will facilitate understanding their form and function. |
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| Keywords: | Cicadellidae FISH Leafhopper Symbiotic bacteria |
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