Enhancement of tendon-bone healing with the use of bone morphogenetic protein-2 inserted into the suture anchor hole in a rabbit patellar tendon model |
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| Institution: | 1. Department of Orthopedic Surgery, Korea University College of Medicine, Ansan Hospital, Ansan, Gyeonggi-do, South Korea;2. Department of Orthopedic Surgery, Korea University College of Medicine, Guro Hospital, Seoul, South Korea;3. Department of Orthopedic Surgery, Rare Disease Institute, Guro Hospital, Seoul, South Korea;1. Department of Pediatric Hematology/Oncology, IRCCS Bambino Gesù Children''s Hospital, Rome, Italy;2. University of Pavia, Pavia, Italy;3. Department of Biomedical, Surgical, Dental Sciences, University of Milan, Italy;4. UCV Neurobiology, IRCCS Neurological Institute, Carlo Besta, Milan, Italy;1. Pediatric Onco-Hematology, Stem Cell Transplantation and Cellular Therapy Division, City of Science and Health of Turin, Regina Margherita Children''s Hospital, Turin, Italy;2. Department of Public Health and Pediatrics, University of Turin, Turin, Italy;3. Blood Component Production and Validation Center, City of Science and Health of Turin, S. Anna Hospital, Turin, Italy;1. Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Zhejiang 310000, China;2. Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province, School of Medicine, Zhejiang University, China;3. Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, China;4. Orthopaedics Research Institute of Zhejiang University, China;5. Department of Sports Medicine, School of Medicine, Zhejiang University, China;6. Department of Endodontology, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong;7. Department of Biological Sciences, Faculty of Science and Technology, Sunway University, Bandar Sunway, Selangor Darul Ehsan, Malaysia;8. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, 310003 Hangzhou, China;9. Laboratory of Biomechanical Orthopedics, EPFL, Lausanne, Switzerland;10. China Orthopaedic Regenerative Medicine (CORMed), Hangzhou, China |
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| Abstract: | Background aimsSuture anchor fixation failure has been reported as a result of anchor loosening and migration during the tendon-bone repair. The aim of this study was to evaluate the effects of bone morphogenetic protein-2 (BMP-2) inserted into the suture anchor hole on bone formation and the tendon-bone healing.MethodsBoth back legs of 24 New Zealand White rabbits (n = 48) were used in this study. A metal suture anchor was then placed 5 mm below the cortex. In the control group, the space over the eyelet of the anchor (suture anchor hole) was not filled. In the experimental group, the suture anchor hole was filled with 0.1 mL of fibrin glue (group 2) or collagen gel (group 3) with 1 μg BMP-2. Histologic analysis, real-time-polymerase chain reaction, bone density and failure load measurement were performed, and differences were analyzed at 4 and 8 weeks.ResultsHistologic analysis revealed more abundant new bone, mature bone and organized fibrocartilage at the tendon-bone interface at 4 and 8 weeks in groups in which BMP-2 was applied. At 8 weeks, the failure load of groups 1, 2 and 3 was significantly different among the three groups (P = 0.01). After post hoc Tukey test, the failure load of group 2 was significantly higher than that of group 1 (P = 0.01).ConclusionsBMP-2, administrated as described in this study, improved tendon-bone healing and bone formation, resulting in improved biomechanical strength of the tendon-bone junction. |
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| Keywords: | bone morphogenetic protein-2 collagen gel fibrin glue patellar tendon suture anchor |
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