Influence of the Paracoccidioides brasiliensis 14-3-3
and gp43 proteins on the induction of apoptosis in A549 epithelial
cells |
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Authors: | Julhiany de Fátima da Silva Juliana Vicentim Haroldo Cesar de Oliveira Caroline Maria Marcos Patricia Akemi Assato Patrícia Ferrari Andreotti Juliana Leal Monteiro da Silva Christiane Pienna Soares Gil Benard Ana Marisa Fusco Almeida Maria José Soares Mendes-Giannini |
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Institution: | 1.Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista, Araraquara, SP, Brasil;2.Laboratório de Alergia e Imunologia Clínica e Experimental;3.Clínica de Doenças Infecciosas e Parasitárias, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil |
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Abstract: | The fungal strain Paracoccidioides brasiliensis remains viable
inside of epithelial cells and can induce apoptosis in this population. However,
until now, the molecules that participate in this process remained unknown. Thus,
this study evaluated the contribution of two P. brasiliensis
molecules, the 14-3-3 and glycoprotein of 43 kDa proteins, which had been previously
described as extracellular matrix adhesins and apoptosis inductors in human
pneumocytes. Accordingly, epithelial cells were treated with these molecules for
different periods of time and the expression of the apoptosis regulating-proteins
Bak, Bax, Bcl-2, p53 and caspases were evaluated by terminal deoxynucleotidyl
transferase dUTP nick end labelling, flow cytometry and real-time polymerase chain
reaction analysis. Our results demonstrated that treatment with these molecules
induces apoptosis signalling in pulmonary epithelial cells, showing the same pattern
of programmed cell-death as that observed during infection with P.
brasiliensis. Thus, we could conclude that P.
brasiliensis uses these molecules as virulence factors that participate
not only in the fungal adhesion process to host cells, but also in other important
cellular mechanisms such as apoptosis. |
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Keywords: | Paracoccidioides brasiliensis adhesins apoptosis |
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