Impact of chronicity of injury on the proportion of mesenchymal stromal cells derived from anterior cruciate ligaments |
| |
| Institution: | 1. Department of Orthopedic Surgery, Korea University College of Medicine, Seoul, Korea;2. Department of Biochemistry and Molecular Biology, Division of Brain Korea 21 Program for Biomedical Science, Korea University College of Medicine, Seoul, Korea;3. Barunmadi Orthopedics, Seongnam, Gyeonggi-do, Korea;1. Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan;2. Division of Oral and Maxillofacial Surgery, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan;3. Division of Periodontology, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan;4. Midwest Biomedical Research Foundation, Research Service, Department of Veterans Affairs Medical Center, Kansas City, Missouri, USA;5. Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata, Japan;1. Division of Sports Medicine, Department of Orthopedic Surgery, Rush University Medical Center, Chicago, Illinois, U.S.A.;2. Department of Preventative Medicine, Graduate College, Rush University Medical Center, Chicago, Illinois, U.S.A.;3. Department of Radiology, Rush University Medical Center, Chicago, Illinois, U.S.A.;4. Department of General Surgery, William Beaumont Hospital, Royal Oak, Michigan, U.S.A.;5. Department of Orthopaedic Surgery, Naval Medical Center San Diego, San Diego, California, U.S.A.;1. Division of Plastic, Reconstructive and Aesthetic Surgery, Department of Surgery, The Chinese University of Hong Kong, Hong Kong, SAR;2. Department of Obstetrics and Gynaecology, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, SAR;1. Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia;2. Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Lembah Pantai, Kuala Lumpur, Malaysia;3. Department of Orthopedics & Traumatology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia |
| |
| Abstract: | Background aimsThe graft-healing potential of mesenchymal stromal cells (MSCs) derived from the remnants of ruptured anterior cruciate ligaments (ACLs) after ACL reconstruction may depend on the chronicity of the injury. The aim of this study was to assess the quantitative and phenotypic differences between MSCs isolated from ACL remnants in patients with (sub)acute and chronic tearing.MethodsTorn ACL remnants were harvested during ACL reconstruction from 41 patients, 24 with (sub)acute ACL (<6 months from injury to surgery) and 17 with chronic ACL (time interval >6 months) tears. MSCs isolated from these samples were assessed for quantitative and phenotypic differences, and the correlation between the proportion of MSCs and the chronicity of ACL tear was evaluated.ResultsAt passage 0, the mean proportion of MSCs (CD34−, CD44+, CD90+ and CD105+) was higher in (sub)acute than in chronic ACL tear samples (20.69% ± 7.82% versus 9.85% ± 8.01%, P < 0.001). At passages 1 and 2, however, MSC proportions did not differ significantly in the two groups. Time interval showed a negative correlation with MSC proportion only at passage 0 (r = −0.505, P < 0.001). The optimal cutoff value for time from injury to surgery yielding <10% freshly isolated ACL-MSCs, a percentage expected to have low tissue healing potential, was 23.5 months.ConclusionsThe proportion of freshly isolated MSCs was higher in samples from patients with (sub)acute tearing than in chronic ACL tearing and negatively correlated with the time interval between trauma and surgery. |
| |
| Keywords: | acute ACL anterior cruciate ligament chronic ACL mesenchymal stromal cells |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
