Blockage of morphological transformation of chick embryo fibroblasts infected with Rous sarcoma virus by N-myristoyl glycinal diethylacetal in vitro |
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| Authors: | S Shoji M Matsunaga R Tsujita Y Kubota |
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| Affiliation: | Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan. |
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| Abstract: | N-Myristoyl glycinal diethylacetal strongly inhibited morphological transformation of chick embryo fibroblasts infected with a temperature-sensitive mutant (tsNY68) of Rous sarcoma virus. Myristoylated or nonmyristoylated pp60v-src, which were expressed in tsNY68-infected cells in the absence or presence of the compound, were identified separately by fluorography or immunoblotting analysis after sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the [3H]myristate-labeled cell lysate. The results taken together suggest that the blockage of morphological transformation was caused by prevention of protein myristoylation of the transforming protein pp60v-src. |
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