Physiological properties and cytological features of protoplasts prepared fromChlorella saccharophila |
| |
Authors: | R Tischner H Gleibs S Hillmer D G Robinson |
| |
Institution: | (1) Pflanzenphysiologisches Institut, Universität Göttingen, Untere Karspüle 2, D-3400 Göttingen, Germany |
| |
Abstract: | Summary Protoplasts were prepared from cells ofChlorella saccharophila by treatment with a mixture of pectinase and cellulase. The yield of protoplasts is dependent upon the culture conditions prior to cell wall digestion. In thin section chemically-fixed protoplasts were without wall remnants at the surface of the plasma membrane. Of particular interest is the relationship between the Golgi apparatus and a nuclear envelope-endoplasmic reticulum continuum. Protoplasts have a photosynthetic capacity lying between 70 and 80% of that of normal cells, but show the same response towards CO2 concentration and DCMU inhibition. Protoplasts also respond to changes in the osmolarity of the surrounding medium in accordance with the Boylevan't Hoff equation as if they are an osmometer. The nonosmotic volume (NOV) was calculated.Abbreviations GA
Golgi apparatus
- ER
endoplasmic reticulum
- NE
nuclear envelope
- PM
plasma membrane
- N
nucleus
- S
starch
- M
mitochondria
- V
vacuole |
| |
Keywords: | Chlorella Protoplasts Photosynthesis Osmotic properties Nonosmotic volume Ultrastructure |
本文献已被 SpringerLink 等数据库收录! |