Tissue-specific expression of the PAL3 promoter requires the interaction of two conserved cis sequences |
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| Authors: | Diane Hatton Caroline Smith Michael Bevan |
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| Affiliation: | (1) Department of Molecular Genetics, John Innes Centre, Colney Lane, NR4 7UJ Norwich, UK;(2) Present address: Department of Plant Sciences, University of Cambridge, Downing Street, CB2 3EA Cambridge, UK |
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| Abstract: | The bean PAL2 and PAL3 promoters confer expression in overlapping sets of tissue types in transgenic tobacco. The PAL3 promoter contains motifs that resemble two AC cis elements which are required for tissue-specific expression of the PAL2 promoter. The functions of these motifs in the PAL3 promoter were determined by analysis of mutated PAL3 promoter-GUS constructs in transgenic tobacco. This revealed that the AC motifs are necessary for tissue-specific expression of the PAL3 promoter. Therefore, a key role is indicated for AC elements, which are Myb-protein binding sites, in regulating tissue-specific expression of the bean PAL gene family. |
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| Keywords: | cis elements Myb-protein binding sites Phaseolus vulgaris phenylalanine ammonia-lyase tissue-specific expression |
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