In vitro propagation of <Emphasis Type="Italic">Huernia hystrix</Emphasis>: an endangered medicinal and ornamental succulent |
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Authors: | S O Amoo J F Finnie J Van Staden |
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Institution: | (1) Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville, 3209, South Africa |
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Abstract: | An efficient and rapid method for in vitro clonal propagation of Huernia hystrix was developed, resulting in shoot regeneration within 3 weeks of culture. This endangered medicinal and ornamental succulent
is in high demand. Multiple shoots were regenerated from stem explants (10 mm length) cultured on Murashige and Skoog (MS)
medium containing 3% sucrose and supplemented with a range of NAA (0.00–8.06 μM) and BA (4.44–22.19 μM) concentrations. A
100% shoot response with a multiplication rate of four shoots per explant was obtained on MS medium containing 5.37 μM NAA
and 22.19 μM BA. Callus produced at the base of the explant on the same medium showed root organogenic potential. The in vitro
regenerated shoots produced roots when transferred to half strength MS medium with or without auxin. The micropropagated plants
were easily acclimatized within 2 months under greenhouse conditions when potted in a soil and sand mixture (1:1; v/v) treated
with a fungicide (Benlate, 0.01%). More than 95% survival with no observable morphological variations was obtained. The developed
protocol provides a simple, cost-effective means for the conservation of endangered H. hystrix by clonal propagation within a short time. |
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Keywords: | Conservation Micropropagation Succulent Threatened species |
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