Determination of self-locompatibility genotypes of Korean apple cultivars based on S-RNase PCR |
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| Authors: | Hoy Taek Kim Gen Hattori Yutaka Hirata Dae Ill Kim Jeong Hwan Hwang Yong Uk Shin Ill Sup Nou |
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| Institution: | (1) Faculty of Horticulture, Chiba University, 271-8510 Chiba, Japan;(2) United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 183-8509 Tokyo, Japan;(3) Fruit Tree Breeding Division, National Horticultural Research Institute, RDA, 440-706 Suwon, Korea;(4) Faculty of Plant Science and Production, Sunchon National University, 540-742 Sunchon, Korea |
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| Abstract: | To prevent self-fertilization, apple has a gametophytic self-incompatibility mechanism, part of a widespread intraspecific
system, that is controlled by a multi-allelic locus. This attribute has been exploited in breeding programs for new cultivars.
Likewise, many apple orchards depend on artificial pollination. Therefore, molecular analysis and early identification of
the self-incompatibility (S) genotype could greatly improve breeding schemes and pollen donors selection. Here, we PCR-amplified
the S-RNase PCR fragments from a total of 14 cultivars and parents, using new primers (ASPF3+ASPR3) common to 23 S-alleles
in apple. The S-genotypes were determined for the following: ‘Hongro’ (S1S3), ‘Gamhong’ (S1S9), ‘Saenara’ (S1S3), ‘Chukwang’ (S3S9), ‘Hwahong’ (S3S9), ‘Seokwang’ (S3S3), ‘Hwarang’ (S1S9), ‘Sunhong’ (S3S9), ‘S.E.B.’ (S1S19), ‘S.G.D.’ (S2S3), and ‘Mollie’s Delicious’ (S3S7). We also confirmed the characteristics of the S-genotypes for eight Korean apple cultivars by PCR-Southern blot analysis,
using seven S-RNases as probes. |
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| Keywords: | apple gametophytic self-incompatibility PCR S-genotype S-RNase |
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