Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: cloning, sequencing, and characterization |
| |
| Authors: | Basnet Devi Bahdur Oh Tae-Jin Vu Thi Thu Hang Sthapit Basundhara Liou Kwangkyoung Lee Hei Chan Yoo Jin-Cheol Sohng Jae Kyung |
| |
| Institution: | Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, Sun Moon University, Asan 336-708, Korea. |
| |
| Abstract: | The entire gene cluster involved in the biosynthesis of angucyclines Sch 47554 and Sch 47555 was cloned, sequenced, and characterized. Analysis of the nucleotide sequence of genomic DNA spanning 77.5-kb revealed a total of 55 open reading frames, and the deduced products exhibited strong sequence similarities to type II polyketide synthases, deoxysugar biosynthetic enzymes, and a variety of accessory enzymes. The involvement of this gene cluster in the pathway of Sch 47554 and Sch 47555 was confirmed by genetic inactivation of the aromatase, including a portion of the ketoreductase, which was disrupted by inserting the thiostrepton gene. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
