Isolation and identification of 2alpha,25-dihydroxyvitamin D3, a new metabolite from Pseudonocardia autotrophica 100U-19 cells incubated with Vitamin D3 |
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Authors: | Takeda Koji Kominato Kaichiro Sugita Atsuko Iwasaki Yukiko Shimazaki Mika Shimizu Masato |
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Affiliation: | Bioresource Laboratories, Mercian Corporation, Iwata, Shizuoka, Japan. takeda-k@mercian.co.jp |
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Abstract: | Pseudonocardia autotrophica converted Vitamin D(3) to 25-hydroxyvitamin D(3) and 1alpha,25-dihydroxyvitamin D(3). The hydroxylation of Vitamin D(3) with P. autotrophica was enhanced by the addition of cyclodextrin. In this microbial hydroxylation, a new Vitamin D(3) metabolite was observed in the reaction mixture of P. autotrophica and Vitamin D(3), and was isolated in a pure form by several steps of chromatography. The structure of the new metabolite was determined to be 2alpha,25-dihydroxyvitamin D(3) by UV, NMR and mass spectroscopic analyses. Biological evaluation of the new metabolite was conducted by means of several experiments. |
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Keywords: | 2α,25-Dihydroxyvitamin D3 Microbial hydroxylation Pseudonocardia autotrophica Vitamin D receptor Vitamin D-binding protein |
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