| Nuclear assembly of purified Crythecodinium cohnii chromosomes in cell—free extracts of Xenopus laevis eggs |
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| 作者姓名： | Liu XL Shen Y Chen EJ Zhai ZH |
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| 作者单位： | [1]CollegeofLifeSciences,PekingUniversity,Beijing100871,China [2]CollegeofLifeSciences,PekingUniversity,Be |
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| 摘 要： | Incubation of dinoflagellate Crythecodinium cohnii chromosomes in cytoplasmic extracts of unfertilized Xenopus laevis eggs resulted in chromosomes decondensation and recondensation,nuclear envelope assembly,and nuclear reconstitution.Dinoflagellate Crythecodinium cohnii is a kind of primitive eukaryote which possesses numerous permanently condensed chromosomes and discontinuous double-layered nuclear membrane throughout the cell cycle.The assembled nuclei,being surrounded by a continuous double membrane containing nuclear pores and the uniformly dispersed chromatin fibers are morphologically distinguishable from that of Dinoflagellate Crythecodinium cohnii.However,incubation of dinoflagellate Cyrthecodinium cohnii chromosomes in the extracts from dinoflagellate Crythecodinium cohnii cells does not induce nuclear reconstitution.
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| 关 键 词： | 纯化 甲藻染色体 爪蟾 蛙卵无细胞提取物 核重组 细胞核 |
Nuclear assembly of purified Crythecodinium cohnii chromosomes in cell-free extracts of Xenopus laevis eggs |
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| Liu XL,Shen Y,Chen EJ,Zhai ZH.Nuclear assembly of purified Crythecodinium cohnii chromosomes in cell-free extracts of Xenopus laevis eggs[J].Cell Research,2000,10(2):127-137. |
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| Authors: | Liu X L Shen Y Chen E J Zhai Z H |
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| Institution: | College of Life Sciences, Peking University, Beijing, China. |
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| Abstract: | Incubation of dinoflagellate Crythecodinium cohnii chromosomes in cytoplasmic extracts of unfertilized Xenopus laevis eggs resulted in chromosomes decondensation and recondensation, nuclear envelope assembly, and nuclear reconstitution.Dinoflagellate Crythecodinium cohnii is a kind of primitive eukaryote which possesses numerous permanently condensed chromosomes and discontinuous double-layered nuclear membrane throughout the cell cycle. The assembled nuclei, being surrounded by a continuous double membrane containing nuclear pores and the uniformly dispersed chromatin fibers are morphologically distinguishable from that of Dinoflagellate Crythecodinium cohnii. However, incubation of dinoflagellate Crythecodinium cohnii chromosomes in the extracts from dinoflagellate Crythecodinillm cohnii cells does not induce nuclear reconstitution. |
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| Keywords: | Dinoflagellate Crythecodinium cohnii chromosome nuclear reconstitution cell-free system Xenopus laevis egg extracts |
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