Over-expression of the cloned rice thaumatin-like protein (PR-5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease |
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| Authors: | K Datta R Velazhahan N Oliva I Ona T Mew G S Khush S Muthukrishnan S K Datta |
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| Institution: | (1) Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, P.O. Box 3127, 1271 Makati City, Philippines Fax: +63-2-8911292 E-mail: sdatta@irrl.cgnet.com; sdatta@irrl.cgiar.org, XX;(2) Department of Biochemistry, Kansas State University, Manhattan, KS 66506-3702, USA, US;(3) Entomology and Plant Pathology Division, International Rice Research Institute, P.O. Box 933, Manila 1099, Philippines, XX |
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| Abstract: | A 1.1-kb DNA fragment containing the coding region of a thaumatin-like protein (TLP-D34), a member of the PR-5 group, was
cloned into the rice transformation vector pGL2, under the control of the CaMV 35S promoter. The Indica rice cultivars, ‘Chinsurah
Boro II’, ‘IR72’, and ‘IR51500’ were transformed with the tlp gene construct by PEG-mediated direct gene transfer to protoplasts and by biolistic transformation using immature embryos.
The presence of the chimeric gene in T0, T1, and T2 transgenic plants was detected by Southern blot analysis. The presence of the expected 23-kDa TLP in transgenic plants was
confirmed by Western blot analysis and by staining with Coomassie Brilliant Blue. Bioassays of transgenic plants challenged
with the sheath blight pathogen, Rhizoctonia solani, indicated that over-expression of TLP resulted in enhanced resistance compared to control plants.
Received: 11 August 1998 / Accepted: 26 August 1998 |
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| Keywords: | Thaumatin-like protein PR-5 Rhizoctonia solani Sheath blight disease Rice |
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