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The duplicated gene copy of the ovine growth hormone gene contains a PvuII polymorphism in the second intron |
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| Authors: | J. A. Sise J. M. Penty G. W. Montgomery E. Gootwine |
| Affiliation: | AgResearch Molecular Biology Unit, Department of Biochemistry and Center for Gene Research, University of Otago, Dunedin, New Zealand;Institute of Animal Science, The Volcani Center, ARO POB 6 Bet Dagan 50250, Israel |
| Abstract: | PvuII restriction fragment length polymorphism (RFLP) was found at the growth hormone locus in sheep carrying the GH2 allele where the gene is duplicated. By restriction analysis and using the polymerase chain reactibn we demonstrated that this RFLP is due to a mutation at the Pvu II site located in the second intron of the 3′ copy of the GH2 allele. |
| Keywords: | ovine growth hormone RFLP PCR |