Determination of the complete nucleotide sequence of pNS1, a staphylococcal tetracycline-resistance plasmid propagated in Bacillus subtilis |
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Authors: | Norihisa Noguchi Takashi Aoki Masanori Sasatsu Megumi Kono Kazuo Shishido Tadahiko Ando |
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Affiliation: | Department of Microbiology, Tokyo College of Pharmacy, Horinouchi, Hachioji, Tokyo 192-03, Japan;Laboratory of Natural Products Chemistry, Tokyo Institute of Technology, Nagatsuta, Yokohama, Kanagawa 227, Japan;Department of Microbiology, Riken Institute, Wako, Saitama 351, Japan |
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Abstract: | Abstract The complete nucleotide sequence of pNS1 (3879 bp), a tetracycline-resistance (TcR) plasmid drived from staphylococcal plasmid pTP5, has been determined and compared with that of the staphylococcal TcR plasmid pT181 [6]. The nucleotide sequences of the 2 plasmids are in agreement, except for 18 nucleotides, but these differences are significant in that they give rise to new open reading frames (ORFs). A short ORF-D is found in the copy control region, and the TcR region contains a single large ORF-A, that encodes the Tet protein (50 kDa). The upstream region of ORF-A contains 3 inverted repeat sequences, which can generate structures very similar in conformation of the structure of the control region of the inducible erythromycin-resistance gene of pE194. |
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Keywords: | Staphylococcus aureus Bacillus subtilis plasmid tetracycline resistance nucleotide sequence |
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