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Use of the glucose oxidase system for estimation of oxygen transfer rate in a solid-state bioreactor
Authors:Hua Zhao  Xiaoyong Zhang  Xiaohong Zhou  Zuohu Li
Institution:

a State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, P.O. Box 353, Beijing 100080, Peoples Republic of China

Abstract:The glucose oxidase system was adapted for estimation of the overall oxygen transfer rate in a periodic pressure oscillating, solid-state bioreactor. Enzyme concentration of 40 ml enzyme preparation L?1 was found adequate to give linear gluconic acid production and attain maximal oxygen absorption rates. At 4 atm and 30°C, the oxygen transfer rate reached 892 mmol kg?1 initial dry matter h?1 in this system, while only 121 mmol kg?1 initial dry matter h?1 was obtained in a conventional static tray bioreactor.
Keywords:Glucose oxidase system  Oxygen transfer rate  Periodic pressure oscillation  Solid-state bioreactor
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