| 人骨骼肌M蛋白的提取与纯化 |
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| 引用本文： | 屈竹青,卢鼎厚. 人骨骼肌M蛋白的提取与纯化[J]. 中国生物化学与分子生物学报, 1994, 10(2): 135-139 |
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| 作者姓名： | 屈竹青 卢鼎厚 |
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| 作者单位： | 北京体育学院运动生理教研室 |
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| 摘 要： | 依据Ｔｒｉｎｉｃｋ－Ｅｐｐｅｎｂｅｒｇｅｒ对鸡骨骼肌Ｍ蛋白的提取方法，由人骨骼肌中得到的Ｍ蛋白粗提物除含分子量为１６５０００的Ｍ蛋白外，还含有分子量为１８５０００和１４００００（Ｃ成分）的两组分。由于在粗提物中未发现分子量为９００００的磷酸化酶，我们将最终纯化步骤中的亲和层析改为制备电泳，同样获得了纯化的Ｍ蛋白。
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| 关 键 词： | M－蛋白 人骨骼肌 |
| 收稿时间： | 1994-04-20 |
The Extraction and Purification of Human Skeletal Muscle M-protein |
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| Qu,Zhuqing,Lu,Ding-hou. The Extraction and Purification of Human Skeletal Muscle M-protein[J]. Chinese Journal of Biochemistry and Molecular Biology, 1994, 10(2): 135-139 |
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| Authors: | Qu Zhuqing Lu Ding-hou |
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| Affiliation: | (Section of Exercise Physiology,Beijing Institute of Physical Education,Beijing 100084 |
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| Abstract: | Three protein components were obtained from the crude extract of human skeletal muscle M-protein by Trinick-Eppenberger's method for preparation of M-protein from chicken skeletal muscle.There MW were 165000(M protein), 185000 and 145000 respectively. The MW 185000 may probably be myomesin, but whether the 140000 component is the degraded product of M-protein or some other protein is still not clear. The pure M-protein was obtained by the preparative electrophoresis instead of affinity chromatography. |
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| Keywords: | Human skeletal muscle M-protein |
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