Ontogeny of Glucose Metabolism in Sympathetic Ganglia of Chickens. Changes in the Carbon Fluxes to CO2, Lactate, and Tissue Constituents from 8 to 19 Days of Embryonic Age

Chains of sympathetic ganglia were excised from the lumbar region of white Leghorn chicken embryos, 8-19 days of age. The chains were incubated for 5 h at 36 degrees C in a bicarbonate-buffered physiological salt solution containing 5.55 mM unlabeled glucose and tracer amounts of glucose labeled either uniformly or at carbon-1 or carbon-6. Glucose uptake and labeled lactate output were both highest in ganglia from the youngest embryos studied and declined progressively with increasing age. The output of labeled CO2 rose to a peak rate at an incubation age of 10-12 days in the presence of either U-14C]glucose or 1(-14)C]glucose, but changed relatively little with age in the presence of 6(-14)C]glucose. The incorporation of 14C into tissue constituents was fastest at 10-12 days with all three labeled glucoses. It is concluded that the hexosemonophosphate shunt is most active at an incubation age of 10-12 days, after glycolysis has greatly slowed. The literature on morphological and biochemical changes in the sympathetic ganglia during development is briefly reviewed and discussed in relation to the observed metabolic changes. The early high glycolytic rate may be related to the normal developmental delay in vascularization of the sympathetic chains.