The c15 ring of the Spirulina platensis F-ATP synthase: F1/F0 symmetry mismatch is not obligatory |
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Authors: | Pogoryelov Denys Yu Jinshu Meier Thomas Vonck Janet Dimroth Peter Muller Daniel J |
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Institution: | Institute of Microbiology, Eidgen?ssische Technische Hochschule, Wolfgang-Pauli Strasse 10, Zurich 8093, Switzerland. |
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Abstract: | The oligomeric c ring of the F-ATP synthase from the alkaliphilic cyanobacterium Spirulina platensis was isolated and characterized. Mass spectroscopy analysis indicated a mass of 8,210 Da, reflecting that of a c monomer. The mass increased by 206 Da after treatment with the c-subunit-specific inhibitor dicyclohexylcarbodiimide (DCCD), which indicated modification of the ion-binding carboxylate by DCCD. Atomic force microscopy topographs of c rings from S. platensis showed 15 symmetrically assembled subunits. The c15-mer reported here is the largest c ring that is isolated and does not show the classical c-ring mismatch to the three-fold symmetry of the F1 domain. |
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