Specific progesterone receptors in dimethylbenzanthracene (DMBA)-induced mammary tumors. |
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Authors: | J Asselin F Labrie P A Kelly D Philibert J P Raynaud |
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Affiliation: | 1. Medical Research Council Group in Molecular Endocrinology, Centre Hospitalier de l''Université Laval, Québec, GlV 4G2, Canada;2. Centre de Recherches Roussel-UCLAF, Romainville 93230, France |
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Abstract: | Properties of a progesterone receptor present in the cytosol (105,000 xg supernatant) of dimethylbenzanthracene (DMBA)-induced mammary tumors were studied using the highly potent progestin [3H]R 5020 (17, 21-dimethyl-19-nor-pregna-4, 9-diene-3,20-dione). As shown by sucrose gradient analysis, specific binding of [3H] R 5020 is associated with components migrating at 7-8S and 4S. Low affinity binding of the synthetic progestin is eliminated by treatment with dextran-coated charcoal. [3H] R 5020 binding is highly progestin-specific since it is easily displaced by unlabelled norgestrel, R 5020 and progesterone while estradiol-17beta, dihydrotestosterone, testosterone, testosterone and diethylstilbestrol have much lower activity. Dexamethasone and cortisol have little, if any, effect on [3H] R 5020 binding. |
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