Cell type conversion in a mouse melanoma cell clone |
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Authors: | Seiji Sato Takuji Takeuchi |
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Institution: | (1) Department of Biological Science, College of General Education, Tohoku University, Kawauchi, 980 Sendai, Japan |
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Abstract: | Summary A melanoma cell clone was isolated from cultured B16 mouse melanoma cells. This clone,conv, which was characterized by rounded and spindle-shaped cell morphology, was not highly melanotic under the usual culture
condition but had high tyrosinase (dopa oxidase) activity.
When the cells were seeded to form colonies on a plastic culture dish in Eagle’s minimum essential medium supplemented with
10% bovine calf serum, two kinds of cell types always appeared. One was cytochemically dopa-positive and spindle-shaped (S
type cell) with the same phenotypes as those of the parental cells. The other was dopa-negative and fibroblastlike (F type
cell) containing no melanosomes. It was observed that the conversion from S type to F type occurred with a high frequency.
The conversion from F type to S type also occurred but with a low frequency. |
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Keywords: | cell type conversion B16 mouse melanoma cell shape melanotic expression |
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