| 好氧发酵生产琥珀酸工程菌株的构建 |
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| 引用本文： | 康振,耿艳平,张园园,祁庆生.好氧发酵生产琥珀酸工程菌株的构建[J].生物工程学报,2008,24(12):2081-2085. |
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| 作者姓名： | 康振 耿艳平 张园园 祁庆生 |
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| 作者单位： | 1. 山东大学,微生物技术国家重点实验室,济南,250100
2. 山东大学,微生物技术国家重点实验室,济南,250100;山东大学,国家糖工程技术研究中心,济南,250100 |
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| 基金项目： | 国家高技术研究发展计划(863计划) 项目(No. 2006AA02Z218)和国家重点基础研究发展规划(973项目) 项目(No. 2007CB707803)资助。 |
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| 摘 要： | 通过分析大肠杆菌的碳源代谢途径, 利用基因敲除手段, 以Escherichia coli MG1655为出发菌株, 成功构建了琥珀酸好氧发酵生产工程菌E. coli QZ1111 (MG1655?ptsG?poxB?pta?iclR?sdhA)。检测结果表明该菌株能以葡萄糖为碳源, 在好氧发酵且不表达任何异源基因的条件下大量积累琥珀酸。摇瓶试验证明, 琥珀酸发酵产量达到26.4 g/L, 乙酸盐作为唯一检测到的副产物产量为2.3 g/L。二者浓度比达到11.5:1。
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| 关 键 词： | 大肠杆菌 琥珀酸 代谢工程 |
| 收稿时间： | 2008/9/10 0:00:00 |
Construction of Engineered Escherichia coli for Aerobic Succinate Production |
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| Zhen Kang,Yanping Geng,Yuanyuan Zhang and Qingsheng Qi.Construction of Engineered Escherichia coli for Aerobic Succinate Production[J].Chinese Journal of Biotechnology,2008,24(12):2081-2085. |
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| Authors: | Zhen Kang Yanping Geng Yuanyuan Zhang and Qingsheng Qi |
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| Institution: | State Key Lab of Microbial Technology, Shandong University, Jinan 250100, China;State Key Lab of Microbial Technology, Shandong University, Jinan 250100, China;State Key Lab of Microbial Technology, Shandong University, Jinan 250100, China;State Key Lab of Microbial Technology, Shandong University, Jinan 250100, China; National Glycoengineering Research Center, Shandong University, Jinan 250100, China |
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| Abstract: | Based on carbon metabolic pathway analysis of Escherichia coli MG1655, an aerobic succinate fermentation platform was constructed by knocking out five genes (ptsG, poxB, pta, iclR and sdhA), which was named E. coli QZ1111. Flask cultivation results showed that E. coli QZ1111 could accumulate succinate with a concentration of 26.4 g/L under aerobic conditions. The byproduct acetate was only 2.3 g/L. The production ratio of succinate and acetate reached 11.5:1. |
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| Keywords: | Escherichia coli succinic acid metabolic engineering |
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