Cytological and molecular characterization of a novel monogenic dominant GMS in <Emphasis Type="Italic">Brassica napus</Emphasis> L. |
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Authors: | Dao-Jie Wang Ai-Guang Guo Dian-Rong Li Jian-Hua Tian Fei Huang Gen-Lou Sun |
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Institution: | (1) College of Life Science, Northwest A & F University, Yangling, Shaanxi, 712100, China;(2) Hybrid Rapeseed Research Center of Shaanxi Province, Dali, Shaanxi, 715105, China;(3) Biology Department, Saint Mary’s University, Halifax, Nova Scotia, B3H 3C3, Canada |
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Abstract: | A novel genic male sterile (GMS) line in Brassica napus L., which was identified in 1999, was found to be controlled by a monogenic dominant gene, which we have designated as MDGMS.
The microspores of the MDGMS abort before the degradation of the tapetal cell layer. The F1 fertility from any fertile lines
crossed with MDGMS segregated and the ratio was close to 1:1. Bulked segregation analysis (BSA) was employed to identify random
amplified polymorphic DNA (RAPD) markers linked to the Ms gene in MDGMS. Among 880 random 10-mer oligonucleotide primers screened against the bulk DNA of sterile and fertile, one
primer S243 (5′-CTATGCCGAC-3′) gave a repeatable 1500-bp DNA polymorphic segment S2431500 between the two bulks. Analysis of individual plants of each bulks and other types of GMS and cytoplasmic male sterility
(CMS) lines suggest that the RAPD marker S2431500 is closely linked to the MDGMS locus in rapeseed. This RAPD marker has been converted into sequence characterized amplified
region (SCAR) marker to aid identification of male-fertility genotypes in segregating progenies of MDGMS in marker-assisted
selection (MAS) breeding programs. |
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Keywords: | Brassica napus L MDGMS Microsporogenesis RAPD SCAR |
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