Isolation and characterization of the host protein groE involved in bacteriophage lambda assembly. |
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Authors: | T Hohn B Hohn A Engel M Wurtz P R Smith |
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Institution: | Department of Microbiology Biozentrum of the University Basel Klingelbergstrasse 70 CH-4056 Basel, Switzerland;Department of Cell Biology NYU Medical Center 550 First Avenue New York, N.Y. 10016, U.S.A. |
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Abstract: | The groE protein, which is involved in the morphogenesis of several bacteriophages, was isolated using a hybrid bacteriophage λ strain which overproduces it. The protein was characterized using biophysical methods, electron microscopy and digital image processing. We postulate that the gp groE complex contains 14 subunits in a cylindrical aggregate with 7-fold rotational symmetry. Possible mechanisms are discussed for the action of this complex in phage morphogenesis. |
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