Analysis of polymorphonuclear leukocyte (PMN) migration by the leading-front technique : Random locomotion and chemotaxis |
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Authors: | U. -B. S derstr m, G. Simmingsk ld, B. Norberg, O. B ck,L. Rydgren |
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Affiliation: | 1. Department of Internal Medicine, University of Lund, S-221 85 Lund, Sweden;2. Department of Dermatology, University of Umeå, S-901 87 Umeå, Sweden |
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Abstract: | The present study was designed to elucidate the contribution of non-stimulated random movement, stimulated random movement, antitubulin-resistant chemotaxis and antitubulin-sensitive chemotaxis to the casein-induced PMN migration into a micropore filter, evaluated by the leading-front technique. This analysis was conducted by a simplified test design including PMN migration, (a) without casein; (b) in a gradient of casein; and (c) in casein without gradient. Treatment with the antitubulin SPI (a podophyllotoxin derivative) inhibited PMN migration within a casein gradient down to the level of the stimulated PMN random movement induced by casein. The casein-induced PMN chemotaxis measured by the leading-front technique is thus composed of stimulated random movement and antitubulin-sensitive chemotaxis without evidence of antitubulin-resistant chemotaxis. It is suggested that the anti-inflammatory effects of the antitubulins (colchicine, podophyllotoxin, Vinca alcaloids, griseofulvin) are due to an inhibition of the antitubulin-sensitive chemotaxis. |
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