Occurrence and genotypes of Giardia isolated from lambs in Spain |
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Authors: | Marí a Teresa G mez-Mu oz, Cristina Navarro, Marí a Magdalena Garijo-Toledo, Marí a Auxiliadora Dea-Ayuela, Salceda Fern ndez-Barredo, Marí a Teresa P rez-Gracia, Marí a Victoria Domí nguez-M rquez,Rafael Borr s |
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Affiliation: | María Teresa Gómez-Muñoz, Cristina Navarro, María Magdalena Garijo-Toledo, María Auxiliadora Dea-Ayuela, Salceda Fernández-Barredo, María Teresa Pérez-Gracia, María Victoria Domínguez-Márquez,Rafael Borrás |
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Abstract: | Three hundred and eighty six faecal specimens were randomly collected from 1- to 3-month-old lambs from 16 farms in Spain to investigate the presence of different genotypes of Giardia duodenalis. Individual specimens were examined by IFA (Immunofluorescence assay) and β-giardin PCR polymerase chain reaction. Cysts of G. duodenalis were shed by lambs in every flock analyzed, showing a prevalence by farms of 100%. The average prevalence of G. duodenalis for the 386 specimens was 42%, ranging from 8.3 to 80% depending on the farm. β-giardin PCR positive samples were sequenced to determine the genotypes present at each farm and seven new subtypes of β-giardin Assemblage E are reported in this study. In each farm, one to six different β-giardin subtypes were found, showing the high variability of the target. Also, one flock had the zoonotic Assemblage A. This is the first report of Giardia subgenotype A-1 in sheep in Spain. |
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Keywords: | Giardia Lambs Zoonotic β -Giardin |
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