Alpha-amylase inhibitors from Ficus sp. seeds and their activities towards coleoptera insect pests |
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| Authors: | Bezerra Ingrid W L Teixeira Fabiano M Oliveira Adeliana S Araújo Carina L Leite Edda L Queiroz Karla C S Sales Maurício P |
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| Institution: | Departamento de Bioquimica, Centro de Biociências, Universidade Federal do Rio Grande do Norte, 59072-970, Natal/RN, Brazil. |
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| Abstract: | Alpha-amylase Inhibitors were isolated from Ficus sp. (Gameleira) seeds by acetone fractionation and Sephadex G-50. Two inhibitors (alpha-PPAI and alpha-ZSAI) were tested against alpha-amylases from coleopteran larvae. alpha-PPAI was active to alpha-amylases of Callosobruchus maculatus (52%) and Zabrotes subfasciatus (53%). alpha-ZSAI was strongly active to Z. subfasciatus (100%) of and Mimosestes mimosae (98%). The alpha-ZSAI is a glycoprotein of approximately 50 kDa with an IC50 value of 0.074 microg microl(-1). |
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