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Continuous enzymatic production of lactobionic acid using glucose-fructose oxidoreductase in an ultrafiltration membrane reactor
Authors:M. Satory  M. Fürlinger  D. Haltrich  K.D. Kulbe  F. Pittner  B. Nidetzky
Affiliation:1. Institut für Lebensmitteltechnologie, Universit?t für Bodenkultur Wien, A-1190, Wien, Austria
2. Institut für Biochemie und molekulare Zellbiologie und Ludwig Boltzmann Forschungsstelle für Biochemie, Universit?t Wien, A-1030, Wien, Austria
Abstract:Glucose-fructose oxidoreductase from Zymomonas mobilis catalyzed the oxidation of various aldose sugars to the corresponding aldonic acids. The enzyme was used for the selective and high-yield conversion of lactose to lactobionic acid in batch, fed-batch and continous reaction mode. A productivity of 110 g L d was obtained in an ultrafiltration membrane reactor, operated for 70 h.
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