ELISA – Nachweis von Pflanzenviren mit Hilfe unterschiedlicher Reagenzstreifen |
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| Authors: | D. Reichenbä cher,E. Reiss,Hans-D. Hunger, J. Richter |
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| Affiliation: | Institut für Phytopathologie der Akademie der Landwirtschaftswissenschaften der DDR —4320 Ascherslehen, Zentralinstitut fur Molekularhiologie der Akademie der Wissenschaften der DDR —1115 Berlin-Buch |
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| Abstract: | Abstract Detection of plant viruses by ELISA using different reagent strips
A simplified immunoassay for detection of plant viruses under field conditions was developed on the basis of the direct double antibody sandwich ELISA using dry reagent carriers immobilized on PVC-supports which are arranged in a fan-like manner. The fan consists of a first reagent carrier with antivirus IgG covalently bound to cyanuric chloride-activated (CCA) paper while the second and third are filter papers impregnated with alkaline phosphatase labelled antivirus IgG and the fluorogenic subsrate, 4-methylumbelliferylphosphate, respectively. Performing the test, the first carrier is contacted with a liquid sample containing the virus to be identified, the second and third carriers is contacted with a liquid sample containing the virus to be identified the second and third carriers are sequentially put one upon the other and the reactions carried out. The virus is detected by the reacted substrate fluorescing under a UV-light. The applicability of the test is demonstrated with cucumber mosaic virus and potato virus X. |
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