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Role of inhibitors in the induction of differentiation in callus cultures of Brassica,Datura and Nicotiana
Authors:Urmil Sethi  Atanu Basu  Sipra Guha Mukherjee
Institution:(1) Plant Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110067 New Delhi, India
Abstract:The effect of various inhibitors on differentiation (shoot morphogenesis) in callus cultures of Brassica, Datura and Nicotiana has been investigated. Hormone medium without any inhibitor (control), resulted in 6% shoot formation. Addition of inhibitors such as actinomycin D, cordycepin, abscisic acid, trigonelline and theophylline greatly enhanced shoot formation. The results suggest that inhibitors play a regulatory role in the control of differentiation.Abbreviations ABA Abscisic acid - BA Benzyl adenine - Kn Kinetin - MS Murashige Skoog's - NAA 1-naphthalene acetic acid
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