Role of inhibitors in the induction of differentiation in callus cultures of Brassica,Datura and Nicotiana |
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Authors: | Urmil Sethi Atanu Basu Sipra Guha Mukherjee |
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Institution: | (1) Plant Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110067 New Delhi, India |
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Abstract: | The effect of various inhibitors on differentiation (shoot morphogenesis) in callus cultures of Brassica, Datura and Nicotiana has been investigated. Hormone medium without any inhibitor (control), resulted in 6% shoot formation. Addition of inhibitors such as actinomycin D, cordycepin, abscisic acid, trigonelline and theophylline greatly enhanced shoot formation. The results suggest that inhibitors play a regulatory role in the control of differentiation.Abbreviations ABA
Abscisic acid
- BA
Benzyl adenine
- Kn
Kinetin
- MS
Murashige Skoog's
- NAA
1-naphthalene acetic acid |
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Keywords: | |
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