Binding of human ribosomal protein p40 and its truncated mutants to the small ribosomal subunit |
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Authors: | O A Kossinova A A Malygin E S Babailova G G Karpova |
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Institution: | (1) Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia |
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Abstract: | Ribosomal protein SA (rpSA), or p40, is a structural element of the small subunit of the eukaryotic ribosome. The N-terminal and central parts of rpSA are homologous to prokaryotic S2, whereas its C-terminal part is specific to eukaryotes. Preparations of 40S ribosomal subunits isolated from full-term human placenta proved to be deficient in SA to a varying extent. To study the rpSA binding to human 40S subunits, recombinant rpSA and its mutant forms with N-and C-terminal deletions were synthesized. The full-size and N-truncated rpSA variants bound to 40S subunits, while deletion of the C-terminal domain completely abolished the binding. |
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Keywords: | ribosomal protein SA (p40) laminin-binding domain 40S ribosomal subunit RNA-protein interaction |
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