Combined effects of somatomedin-like growth factors with fibroblast growth factor or epidermal growth factor in DNA synthesis in rabbit chondrocytes |
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Authors: | Yuji Hiraki Hiroyuki Inoue Yukio Kato Michiko Fukuya Fujio Suzuki PhD |
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Institution: | (1) Department of Biochemistry and Calcified-Tissue Metabolism, Faculty of Dentistry, Osaka University, 1-8, Yamadaoka, 565 Suita, Osaka, Japan;(2) Department of Physiology, Biophysics at Harvard Medical School, 02115 Boston, Massachusetts, USA;(3) Seito Hospital, 565 Suita, Osaka, Japan;(4) Department of Biochemistry, Faculty of Densitry, Osaka University, 1-8, Yamada-oka, 565 Suita, Osaka, Japan;(5) Present address: Department of Orthodontics, Faculty of Dentistry, Osaka University, Japan |
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Abstract: | Summary The somatomedin-like growth factors cartilage-derived factor (CDF) and multiplication-stimulating activity (MSA) stimulate DNA synthesis and proliferation of rabbit costal chondrocytes under serum-free conditions. Previously, we suggeted that CDF and MSA act on chondrocytes in an early G1 phase to stimulate DNA synthesis. CDF and MSA have synergistic effects with epidermal growth factor (EGF) or fibroblast growth factor (FGF) in stimulating DNA synthesis of the cells. The mode of combined action of CDF or MSA with EGF or FGF in chondrocytes was studied by sequential treatments with these agents. EGF or FGF had synergistic effects with CDF or MSA in stimulating DNA synthesis, even when added 10 h after the latter. Synergism was also observed in cells pretreated with CDF or MSA; That is, the cultures were treated for 5 h with CDF or MSA and then washed, and treated with FGF or EGF. However, when CDF or MSA was added more than 5 h after EGF or FGF, no synergism of effects was observed. These findings suggest that the cultured chondrocytes become activated to interact with FGF or EGF for commitment to DNA synthesis when they are exposed to somatomedin-like growth factors at an early stage in the G1 phase. Thus chondrocytes are under a different mechanism of growth control from fibroblastic cells.Abbreviations CDF
cartilage-derived factor
- MSA
multiplication-stimulating activity
- EGF
epidermal growth factor
- FGF
fibroblast growth factor |
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Keywords: | cartilage-derived factor multiplication-stimulating activity fibroblast growth factor epidermal growth factor cell cycle traverse |
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