Analysis of the Campylobacter jejuni Genome by SMRT DNA Sequencing Identifies Restriction-Modification Motifs |
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| Authors: | Jason L O’Loughlin Tyson P Eucker Juan D Chavez Derrick R Samuelson Jason Neal-McKinney Christopher R Gourley James E Bruce Michael E Konkel |
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| Institution: | 1School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, United States of America;2Department of Genome Sciences, University of Washington, Seattle, Washington, United States of America;Iowa State University, UNITED STATES |
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| Abstract: | Campylobacter jejuni is a leading bacterial cause of human gastroenteritis. The goal of this study was to analyze the C. jejuni F38011 strain, recovered from an individual with severe enteritis, at a genomic and proteomic level to gain insight into microbial processes. The C. jejuni F38011 genome is comprised of 1,691,939 bp, with a mol.% (G+C) content of 30.5%. PacBio sequencing coupled with REBASE analysis was used to predict C. jejuni F38011 genomic sites and enzymes that may be involved in DNA restriction-modification. A total of five putative methylation motifs were identified as well as the C. jejuni enzymes that could be responsible for the modifications. Peptides corresponding to the deduced amino acid sequence of the C. jejuni enzymes were identified using proteomics. This work sets the stage for studies to dissect the precise functions of the C. jejuni putative restriction-modification enzymes. Taken together, the data generated in this study contributes to our knowledge of the genomic content, methylation profile, and encoding capacity of C. jejuni. |
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