| 水稻原生质体再生成熟植株 |
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| 引用本文： | 王光远,夏镇澳. 水稻原生质体再生成熟植株[J]. 分子细胞生物学报, 1987, 0(2) |
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| 作者姓名： | 王光远 夏镇澳 |
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| 作者单位： | 中国科学院上海植物生理研究所(王光远)，中国科学院上海植物生理研究所(夏镇澳) |
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| 摘 要： | 水稻原生质体培养一直是人们注意的问题,近年来这方面工作开始有了可喜的进展。本文简要报道用水稻种子成熟胚诱发的愈伤组织所制备的原生质体,经离体培养得到再生成熟植株的结果。
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MATURE PLANT REGENERATION FROM RICE PROTOPLASTS |
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| Wang Guang-yuan Xia Zhen-ao. MATURE PLANT REGENERATION FROM RICE PROTOPLASTS[J]. Journal of Molecular Cell Biology, 1987, 0(2) |
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| Authors: | Wang Guang-yuan Xia Zhen-ao |
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| Abstract: | Seeds of rice (Oryza sativa L.) were cul-tured on MS solid medium (2,4-D 2 mg/1).From mature embryos,the calli were induced.After subculture,thick and globular calliwere selected.Protoplasts were isolated fromthese calli and then were cultured in N_4 me-dium (2,4-D 2,6-BA 0.2mg/1).The pro-toplasts grew,repeatly divided and formedcalli.After inducing differentiation regenerat-ed mature plants were obtained. |
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| Keywords: | Oryza sativa Protoplast culture.Mature plant regeneration. |
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