禾谷镰孢菌Fusarium graminearum Schwabe分生孢子萌发过程中的核相变化及有丝分裂过程观察

通过Giemsa染色观察禾谷镰孢菌Fusarium graminearum分生孢子萌发过程中的核相变化及有丝分裂过程。观察表明,分生孢子细胞为单核,细胞核在分生孢子细胞内分裂后进入芽管,在芽管内进行多次分裂,使芽管内细胞核数目不断变化。禾谷镰孢菌有丝分裂过程可以分为4个时期,前期染色体逐渐浓缩变短,中期染色体清晰可见,后期染色单体发生分离并向相反的两极移动,末期形成新的子核。有丝分裂过程中染色体的分离同步或不同步,不同步分离中的滞后染色体形成后期桥的现象更为普遍。

Mitotic division in germ tube of Fusarium graminearum Schwabe

The nuclear phase and mitosis in germ tube of Fusarium graminearum were studied with staining by Giemsa techniques. There was single nucleus in conidial cell. After division in conidial cell, the nuclei entered into germ tube and divided repeatedly, and therefore the number of nuclei in germ tube variably multiplied. The mitosis of F. graminearum has four phases. The chromosomes gradually shortened and thickened during prophase, and at metaphase the chromosomes were distinctly visible. The chromatids separated at anaphase and moved to opposite poles, and then, daughter nuclei were shaped at telophase. Synchronous and asynchronous chromosome separation was seen, and it was more common that the lagging chromosomes formed anaphase bridges.