鲢,鳙,银鲫和团头鲂肠道内分泌细胞中3种肽激素的免疫组化研究

应用ＡＢＣ免疫组化技术，用抑胃多肽、５－羟色胺和内啡肽３种哺乳动物抗血清对鲢、鳙、银鲫和团头鲂的肠粘膜中内分泌细胞进行了检测。结果４种鱼的肠粘膜中均有抑胃多肽免疫反应内分泌细胞存在，但未发现５－羟色胺和内啡肽的免疫反应。抑胃多肽免疫反应内分泌细胞主要分布在前肠前段，并单个地散布在肠褶顶部上皮细胞与杯状细胞之间，多呈长梭形。本文比较了不同食性鱼类和其他动物肠道抑胃多肽免疫反应内分泌细胞的分布规律，讨论了用５－羟色胺和内啡肽免疫染色的结果。

IMMUNOHISTOCHEMICAL STUDIES OF 3 KINDS OF PEPTIDE HORMONES IIN ENDOCRINE CELLS OF THE GUT OF SILVER CARP, BIGHEAD, SILVER CRUCIAN CARP AND BLUNT-SNOUT BREAM

Using immunohistochemical staining technique of avidin-biotin-peroxidase complex (ABC) method, gastric inhibitory polypeptide (GIP), 5-hy-droxytryptamine(5-HT) and endorphine(END) antisera raised against mammalian hormones were employed to investigate the distribution of endocrine cells in the intestinal mucosa of silver carp (Hypophthalmichthys molitrix), bighead (Aristichthys nobilis), silver crucian carp (Carassius gibelio) and blunt-snout bream (Megalobrama amblycephala).GIP-immunoreactive endocrine cells were found in the intestinal mucosa of the 4 species of fishes. But immunoreactive endocrine cells of 5-HT and END were not found.GIP-immunoreactive endocrine cells are distributed mostly in the anterior segment of the foregut, and located mostly in the apical parts of the gut folds and dispersed between the epithelial cells and goblet cells. Most of GIP-immunoreactive endocrine cells were long shuttle shaped. The distributive laws of GIP-immunoreactive endocrine cells in the intestinum of fishes were compared with of different feeding habits other animals. The immunostaining results are discussed in relation to the use of 5-HT and END antisera.