A leucyl-transfer RNA specified by the amber suppressor gene Su6+ |
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| Authors: | K P Gopinathan A Garen |
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| Affiliation: | 1. Department of Chemistry, Faculty of Science, University of Zagreb, Zagreb 10000, Croatia;2. Proteome Center Tuebingen, University of Tuebingen, Tuebingen 72076, Germany;3. Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, and the MRC London Institute of Medical Sciences, London, W12 0NN, United Kingdom;4. Department of Biomolecular Sciences, Weizmann Institute of Science, Rehovot 76100, Israel;5. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany;1. Advanced Manufacturing Laboratory, Industrial Engineering Department, Sharif University of Technology, Tehran, Iran |
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| Abstract: | The species of leucyl-tRNA responsible for the amber suppressor activity of the Su6+ gene was partially purified (60-fold increase in specific suppressor activity) by chromatography on benzoylated DEAE-cellulose. The homologous species of leucyl-tRNA (without suppressor activity) was similarly purified from an Su6− strain. Each of the species of tRNA accounts for about 5% of the total leucine-acceptor activity. The binding to ribosomes of leucyl-suppressor tRNA from the Su6+ strain is stimulated by the amber codon UAG but not by the leucine codon UUG. The homologous leucyl-tRNA from the Su6− strain shows the opposite codon specificity, binding to ribosomes with UUG but not with UAG, These results suggest that in the Su6+ strain, the anticodon CAA of one species of leucyl-tRNA has been changed to CUA as a result of the suppressor mutation in the Su6 gene. There is another species of leucyl-tRNA which also binds to ribosomes with UUG; this species is not affected by the suppressor mutation and therefore remains available in the Su6+ strain for the translation of UUG. |
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