Modulation of a thermoregulated type VI secretion system by AHL-dependent Quorum Sensing in <Emphasis Type="Italic">Yersinia pseudotuberculosis</Emphasis> |
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Authors: | Weipeng?Zhang Shengjuan?Xu Jing?Li Xihui?Shen Email author" target="_blank">Yao?WangEmail author Email author" target="_blank">Zhiming?YuanEmail author |
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Institution: | (1) Wuhan Institute of Virology, Chinese Academy of Sciences, 430071 Wuhan, China;(2) Graduate School of the Chinese Academy of Sciences, 100049 Beijing, China; |
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Abstract: | The type VI secretion system (T6SS) is a novel secretion system found in many Gram-negative bacterial pathogens, which appears
to be tightly regulated by different regulatory mechanisms. In the present study, we identified 4 T6SS clusters in Yersinia pseudotuberculosis and demonstrated that they were differentially thermoregulated. Among them, T6SS4 was preferentially expressed at 26°C, and
its expression was growth phase dependent and subject to quorum sensing regulation. Both YpsI and YtbI AHL synthases contributed
to the positive regulation of T6SS4, whereas YpsI synthase played the major role as T6SS4 expression was reduced strongly
in the ypsI mutant strain but weakly in the ytbI mutant strain. Moreover, we provided evidence that exogenous addition of different synthetic AHLs complemented T6SS4 expression
in different efficiencies in an ypsIytbI double mutant strain, suggesting C6-HSL had an antagonistic effect on T6SS4 expression. This is the first study demonstrating
that the expression of T6SS is precisely regulated by temperature, growth phase, and AHL-dependent quorum sensing systems
in Y. pseudotuberculosis. |
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