A target function for quaternary structural refinement from small angle scattering and NMR orientational restraints |
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Authors: | Frank Gabel Bernd Simon Michael Sattler |
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Institution: | (1) Structural and Computational Biology Group, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany |
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Abstract: | We present a novel target function based on atomic coordinates that permits quaternary structural refinement of multi-domain
protein–protein or protein–RNA complexes. It requires that the high-resolution structures of the individual domains are known
and that small angle scattering (SAS) data as well as NMR orientational restraints from residual dipolar couplings (RDCs)
of the complex are available. We show that, when used in combination, the translational and rotational restraints contained
in SAS intensities and RDCs, respectively, define a target potential function that permits to determine the overall topology
of complexes made up of domains with low internal symmetry. We apply the target function on a modestly anisotropic model system,
the Barnase/Barstar complex, and discuss factors that influence the structural refinement such as data errors and the geometrical
properties of the individual domains. |
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Keywords: | Small angle scattering NMR Residual dipolar couplings Macromolecular complex Annealing |
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