Rapid screening of Leuconostoc mesenteroides mutants for elevated proportions of alternan to dextran |
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Authors: | Timothy D. Leathers G. Thomas Hayman Gregory L. Cote |
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Affiliation: | (1) Biopolymer Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, 61604 Peoria, IL, USA;(2) Present address: Phytoproducts Research Unit, NCAUR, ARS, USDA, 61604 Peoria, IL, USA |
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Abstract: | Certain isolates of the bacterium Leuconostoc mesenteroides, such as strain NRRL B-1355, have been found to produce alternan, a polysaccharide with unique properties of potentially high commercial value. However, all of these isolates also produce significant amounts of the polysaccharide dextran, which would be costly to separate from alternan on a commercial basis. We developed a rapid screening method for the isolation of L. mesenteroides mutants that produce elevated proportions of alternan to dextran. With this technique, a set of mutants of strain NRRL B-1355 was isolated, including strain NRRL B-21138, which produced a high proportion of alternan to dextran and showed complete genetic stability after more than 60 generations. |
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