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Ten novel tetranucleotide microsatellite DNA markers from Asiatic black bear, Ursus thibetanus |
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| Authors: | Chih-Chin Shih Chuan-Chin Huang Shou-Hsien Li Mei-Hsiu Hwang Ling-Ling Lee |
| Institution: | (1) Institute of Ecology and Evolutionary Biology, National Taiwan University, Taipei, 106, Taiwan, ROC;(2) Conservation and Research Center, Taipei Zoo, Taipei, 116, Taiwan, ROC;(3) Department of Life Science, National Taiwan Normal University, Taipei, 116, Taiwan, ROC;(4) Institute of Wildlife Conservation, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan, ROC; |
| Abstract: | Ten polymorphic microsatellite markers were developed for the endangered Formosan black bear (Ursus thibetanus formosanus) from a partial genomic library enriched for GAAA repeat. Polymorphism of these loci was evaluated in 27 Formosan black bear specimens of unknown relationship. The number of alleles per locus ranged from 5 to 15 and the observed heterozygosity of each locus ranged from 0.556 to 0.889. These loci should provide useful molecular tools to study conservation genetics of the Formosan black bear and other Asiatic black bears. |
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