Catalytic Properties of Cytoplasmic and Mitochondrial Aconitate Hydratase from Rat Cardiomyocytes |
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Authors: | L V Medvedeva T N Popova V G Artyukhov L V Matasova |
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Institution: | (1) Voronezh State University, Universitetskaya pl. 1, Voronezh, 394003, Russia |
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Abstract: | Enzymatic activity of aconitate hydratase (aconitase, EC 4.2.1.3) from the rat heart is localized in the cytoplasm (65%) and mitochondria (35%). Cytoplasmic and mitochondrial forms of aconitate hydratase were separated by ion-exchange chromatography on CM-Cellulose and CM-Sephadex. The two forms have similar molecular weight, optimal pH range, and spectral properties; however, they have different chromatography properties, K
m for citrate and isocitrate, as well as sensitivity to Fe2+ ions. |
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