Bluetongue virus: Detection of antiviral immunoglobulin G by means of enzyme-linked immunosorbent assay |
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Authors: | JaRue S Manning Martin F Chen |
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Institution: | (1) Department of Bacteriology, University of California, 95616 Davis, California, USA;(2) Department of Microbiology, Oregon State University, 97330 Corvallis, Oregon, USA |
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Abstract: | An enzyme-linked immunosorbent assay was developed to detect antiviral IgG in the sere of sheep exposed to bluetongue virus.
It was found that the enzyme-linked immunosorbent assay is a rapid and sensitive method for the detection of anti-bluetongue
virus antibody. Bluetongue virus antigen prepared from extracts of virus infected BHK and Vero cells were equally effective.
Antigen prepared from uninfected cells when used as coating antigen did not bind IgG from either exposed or unexposed animals.
Sera raised against each of the four individual BTV serotypes, 10, 11, 13, and 17, found in the United States reacted equally
with all four bluetongue virus serotype antigen preparations. Thus, any of the four serotypes can be used as the bluetongue
virus antigen for the detection of anti-bluetongue virus antibody in the bluetongue virus-enzymelinked immunosorbent assay
system. Antiviral IgG was readily detectable 6 days postinoculation. The anti-bluetongue virus antibody concentration continued
to increase through the 35-day postinoculation test period. At 35 days postexposure, antibody titers of 1:1,600 to >1:3,200
were found. The rapid and sensitive nature of the bluetongue virus enzyme-linked immunosorbent assay indicates that this system
should significantly extend serological studies on bluetongue virus. |
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