Isolation of functional RNA from small amounts of different grape and apple tissues |
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Authors: | Claudio Moser Pamela Gatto Mirko Moser Massimo Pindo Riccardo Velasco |
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Institution: | (1) Istituto Agrario di S. Michele all’ Adige, S. Michele a/Adige, I-38010 Trento, Italy |
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Abstract: | An efficient, simple, and small-scale procedure for isolating functional ribonucleic acid (RNA) was successfully applied to
many different tissues of grape and apple. These woody plants are rich in polyphenolic compounds and polysaccharides that
could impair the RNA extraction. The method chosen is based on the use of hot borate buffer at alkaline pH supplemented with
several adjuvants and followed by selective precipitations. Starting with only 0.4 g of fresh tissue and working with small
tubes (2 mL), we were able to obtain good yields of high-quality RNA suitable for further applications. The procedure can
be proposed for many applications, and it is particularly highly recommended when isolating RNA from a large number of samples. |
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Keywords: | Apple grape hot borate RNA contamination RNA extraction |
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