Novel method for preparing spheroplasts from cells with an internal cellulosic cell wall |
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| Authors: | Kwok Alvin C M Mak Carmen C M Wong Francis T W Wong Joseph T Y |
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| Affiliation: | Department of Biology, Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, People's Republic of China. |
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| Abstract: | Protoplast and spheroplast preparations allow the transfer of macromolecules into cells and provide the basis for the generation of engineered organisms. Crypthecodinium cohnii cells harvested from polyethylene glycol-containing agar plates possessed significantly lower levels of cellulose in their cortical layers, which facilitated the delivery of fluorescence-labeled oligonucleotides into these cells. |
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