High-performance liquid chromatographic-electrospray mass spectrometric determination of morphine and its 3- and 6-glucuronides: application to pharmacokinetic studies |
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| Authors: | R Pacifici S Pichini I Altieri A Caronna A R Passa P Zuccaro |
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| Institution: | 1. Department of Analytical Chemistry, Nutrición y Bromatología, Faculty of Chemistry and Health Research, Institute of Santiago de Compostela (IDIS), University of Santiago de Compostela, Avda de las Ciencias S/N, Santiago de Compostela E-15782, Spain;2. Institute of Legal Medicine, Forensic Toxicology Service, University of Santiago de Compostela, Santiago de Compostela E-15782, Spain;3. Addictive Behaviors Unit of Ourense, Ourense E-32005, Spain;4. Department of Chemistry and Biochemistry, International Forensic Research Institute, Florida International University, Miami, FL 33199, USA;5. Department of Pharmacy, Faculty of Allied Health Sciences, Daffodil International University, Dhaka 1207, Bangladesh |
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| Abstract: | A rapid and selective assay of morphine and its 3- and 6-glucuronides in serum, based on high-performance liquid chromatography-electrospray mass spectrometry has been developed. The analytes and the internal standard, codeine or naltrexone, were subjected to solid-phase extraction, using ethyl solid-phase extraction columns, prior to chromatography. A reversed-phase column and a gradient mobile phase consisting of water and methanol were used. The mass spectrometer was operated in the selected-ion monitoring mode. The following ions were used: m/z 286 for morphine, m/z 300 for codeine, m/z 342 for naltrexone, and m/z 462 for morphine 3- and 6-glucuronides. The limit of quantitation observed with this method was 10 ng/ml morphine, 50 ng/ml morphine-6-glucuronide and 100 ng/ml morphine-3-glucuronide. The present method proved useful for the determination of serum levels of the parent drug and its metabolites in pain patients, heroin addicts and in morphine-treated mice. |
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