培养条件对棘孢曲霉SM-L22所产纤维素酶系组成的影响*

本文研究了影响棘孢曲霉SM-L22纤维素酶系组成的培养条件。研究结果表明,碳源、氮源和初始pH对棘孢曲霉所产生纤维素酶的内、外切酶组分的比例有明显的影响。在2%麸皮,1%CF11,0.5%尿素或含尿素的复合氮源为氮源,初始pH为4.5时,28℃培养120h后,内、外切酶的比值最大,内切酶活可达到3.1 IU/ml,FPA为0.105 IU/ml,CMCase/FPase的比值为30.6。

EFFECTS OF FERMENTATION CONDITIONS ON ASPERGILLUS ACULEATUS SM-L22 CELLULASE COMPOSITION

The effects of fermentation conditions on cellulase composition of Aspergillus aculeatus SM-L22 were studied. The results demonstrated that the carbon sources, nitrogen sources and the initial pH of the media can effect the rate of proportion of CMCase/FPase. The highest proportion of CMCase/FPase was obtained after growing 120 hr under the following culture conditions: 2% wheat bran, 1% cellulose CF11, 0.5% urea or mixed nitrogen resource containing urea and the initial pH of media being 4.5. When endoglucanase (CMCase) activity in the fermented liquid was 3.1 IU/ml and the FPase activity was 0.105 IU/ml, the final CMCase/FPase value reashed 30.6.