Scavenger lipoprotein receptors are more effective in ligand internalization than low density lipoprotein receptors in human monocyte-macrophages |
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Authors: | G A Coetzee A Chait |
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Affiliation: | Division of Metabolism, Endocrinology and Nutrition, RG-26, Department of Medicine, School of Medicine, University of Washington, Seattle, Washington 98195 USA |
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Abstract: | Human monocyte-macrophages in culture express specific receptors for low density lipoproteins (LDL receptor) and human acetylated LDL (AcLDL receptors or scavenger receptors). After 24 h in lipoprotein-deficient serum, the cells expressed 2-3 fold more AcLDL receptors than LDL receptors as measured by trypsin releasable radioactivity after exposure to 125I-LDL or 125I-AcLDL at 37 degrees C. The efficiency of intracellular ligand delivery by the two receptors was evaluated as an internalization index (defined as intracellular + degraded/bound ligand). This index was several fold greater for 125I-AcLDL than for 125I-LDL, in the same cells exposed to either ligand under identical conditions. These results suggest that the scavenger receptors recycle more rapidly than do LDL receptors. |
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Keywords: | LDL low density lipoprotein ACLDL low density lipoprotein modified by acetylation |
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