催产时鳝卵磷酸酶活性的变化及其与排卵的关系

本实验采用Gomori组织化学法和磷酸苯二钠法,分别检测黄鳝卵ALP、ACP的细胞定位和活性变化。结果表明,催产后72h,ALP活性显著高于临近注射时(Oh),催产后48h和96h(p<0.05);临产前(催产后96h),ACP活性极显著高于试验组其它各时程(p<0.01)。提示ALP和ACP活性增强与卵母细胞成熟、排放有直接关系。本研究为探索排卵机制提供资料。

RELATIONSHIP BETWEEN OOCYTE PHOSPHATASE ACTIVITY AND OVULATION AFTER INDUCED SPAWNING IN MONOPTERUS ALBUS

Ninety sexually mature fish (Monopterus albus) were selected and divided into the experimental and control group. After LHRH-A was injected, the enzyme sites and changes in the activity of the alkaline phosphatase (ALP) and acid phosphatase (ACP) in the oocytes of M. albus were determined using Gomori histochemical method and king-Altman method, respectively. The result shows that ALP is located in the folliculi and zona radiata while ACP is located only in the folliculi. Whan the oocytes are reaching final maturation (72 hours after injection), ALP activity in the experimental group is significantly higher than that in the control group (p<0.01). In the experimental group, the ALP activity of 72 h is also significantly higher than that at 0, 48 and 96 h after injection (p<0.05). Prior to spawning (96 hours after injection),ACP activity in the experimental group is higher than that in the control group (p<0.01); it is also significantly higher than that in the experimental group at other times (p<0.01). Both ALP activity and ACP activity decrease significantly after spawning (p<0.05). It is suggested that the increase in ALP and ACP activity bears direct relationship to the maturation and spawning of oocytes after injection.